AffyDEGFinder

WARNING

Given Affymetrix CEL files, AffyDEGfinder finds differentially expressed genes and runs clustering, annotation, and GO and KEGG enrichment analysis for them using R/Bioconductor through Chipster. This workflow consists of the following steps:

  • Normalize the data using rma (normalized.tsv)
  • Run a statistical test on the normalized data using empirical bayes (two-sample.tsv)
  • Perform hierarchical clustering (hc-genes.txt, hc-chips.txt)
  • Write out a heatmap image (heatmap.png)
  • Annotate results (annotations.tsv)
  • Run the term enrichment analysis for KEGG and GO pathways (hypergeo.html)
A description of the WSDL service:

Chipster

Namespace: http://chipster.csc.fi/chipster


  • Port: ChipsterPort
  • Location: http://chipster.csc.fi:80/chipster-ws/chipster
  • Protocol: http://schemas.xmlsoap.org/soap/http
  • Default Style: document

Operations

AffyDEGFinder

  • SOAP Action: http://www.csc.fi/chipster/AffyDEGFinder
  • Input Message: AffyDEGFinder
    • parameters
      • AffyDEGFinder type AffyDEGFinderRequest
        • groups type GroupType
          • files type FileType
            • name type string
              • contents type base64Binary
                • url type string
        • Output Message: AffyDEGFinderResponse
          • parameters
            • AffyDEGFinderResponse type AffyDEGFinderResponse
              • normalizedData type FileType
                • name type string
                  • contents type base64Binary
                    • url type string
                    • deg type FileType
                      • name type string
                        • contents type base64Binary
                          • url type string
                          • geneClustering type FileType
                            • name type string
                              • contents type base64Binary
                                • url type string
                                • chipClustering type FileType
                                  • name type string
                                    • contents type base64Binary
                                      • url type string
                                      • heatmap type FileType
                                        • name type string
                                          • contents type base64Binary
                                            • url type string
                                            • annotations type FileType
                                              • name type string
                                                • contents type base64Binary
                                                  • url type string
                                                  • termEnrichmentAnalysis type FileType
                                                    • name type string
                                                      • contents type base64Binary
                                                        • url type string

                                                  WSDL Changes

                                                  DateDescription of Changes
                                                  8th April 2009First Snapshot
                                                  WARNING

                                                  Test #1: AffyDEGFinder Java test

                                                  Run AffyDEGFinder for some kidney files using Java and JAX-WS RI.

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